clone m290  (Bio X Cell)

Journal: BMC Immunology

Article Title: DX5 + NKT cells display phenotypical and functional differences between spleen and liver as well as NK1.1 - Balb/c and NK1.1 + C57Bl/6 mice

doi: 10.1186/1471-2172-12-26

Figure Lengend Snippet: Activating markers are expressed differently in spleen and liver DX5 + NKT cells . Detection of NK cell markers confirmed differences between spleen and liver subsets of DX5 + NKT cells in NK1.1 - Balb/c mice. CD94 expression was higher in liver DX5 + NKT cells (A). Furthermore, the liver subset revealed less expression of maturation marker CD103 and CD62L compared to spleen (B). The activating markers CD154 and CD178 were in both freshly-isolated cell subsets only little expressed (C). Upon stimulation spleen DX5 + NKT cells displayed an up-regulation of CD154, whereas the liver subset did not. Results are given as mean + SEM. Experiments were repeated at least three times (* p < 0.05).

Article Snippet: The following reagents were used for cell surface labeling in multiparameter flow cytometric analysis (FACS Calibur, BD Bioscience): FITC or Alexa Fluoar 647-conjugated anti-mouse CD3 (clone: 17A2, rat IgG2b), APC-conjugated anti-mouse-CD4 (clone: GK1.5, rat IgG2b), APC-conjugated anti-mouse-CD8a (clone: 53-6.7, rat IgG2a), PE-conjugated anti-mouse-CD38 (clone: 90, rat IgG2a), PE-conjugated anti-mouse-CD49b (clone: DX5, rat IgM), APC-conjugated anti-mouse-CD62L (clone: MEL-14, rat IgG2a), PE-conjugated anti-mouse-CD103 (clone: M290, rat IgG2a), PE-conjugated anti-mouse-CD178 (Fas-Ligand) (clone: MFL3, hamster IgG1), PE-conjugated anti-mouse-Vβ 8.1, 8.2 TCR (clone: MR5-2, rat IgG2a) all BD Biosciences.

Techniques: Expressing, Marker, Isolation

Journal: BMC Immunology

Article Title: DX5 + NKT cells display phenotypical and functional differences between spleen and liver as well as NK1.1 - Balb/c and NK1.1 + C57Bl/6 mice

doi: 10.1186/1471-2172-12-26

Figure Lengend Snippet: FACS-Analysis of spleen DX5 + NKT cells revealed interstrain differences in several phenotypical markers between NK1.1 - Balb/c and NK1.1 + C57Bl/6 mice . In the NK1.1 + mice strain spleen DX5 + NKT cells expressed less CD4 but more CD8 (A). Upon activation the TCR α/β down-regulation was confirmed. In addition to NK.1.1 more spleen DX5 + NKT cells in C57Bl/6 mice were positive for CD94 (B). Maturation marker CD62L was slightly higher expressed in freshly-isolated spleen DX5 + NKT cells in C57Bl/6 mice compared to Balb/c, whereas CD103 was less (C). Functional marker CD154 and CD178 were only little expressed in freshly-isolated spleen DX5 + NKT cells in both mice strains (D). Upon stimulation spleen DX5 + NKT cells in NK1.1 + C57Bl/6 mice displayed less up-regulation of CD154 compared to Balb/c. Results are given as mean + SEM. Experiments were repeated at least three times (* p < 0.05).

Article Snippet: The following reagents were used for cell surface labeling in multiparameter flow cytometric analysis (FACS Calibur, BD Bioscience): FITC or Alexa Fluoar 647-conjugated anti-mouse CD3 (clone: 17A2, rat IgG2b), APC-conjugated anti-mouse-CD4 (clone: GK1.5, rat IgG2b), APC-conjugated anti-mouse-CD8a (clone: 53-6.7, rat IgG2a), PE-conjugated anti-mouse-CD38 (clone: 90, rat IgG2a), PE-conjugated anti-mouse-CD49b (clone: DX5, rat IgM), APC-conjugated anti-mouse-CD62L (clone: MEL-14, rat IgG2a), PE-conjugated anti-mouse-CD103 (clone: M290, rat IgG2a), PE-conjugated anti-mouse-CD178 (Fas-Ligand) (clone: MFL3, hamster IgG1), PE-conjugated anti-mouse-Vβ 8.1, 8.2 TCR (clone: MR5-2, rat IgG2a) all BD Biosciences.

Techniques: Activation Assay, Marker, Isolation, Functional Assay

Journal: Cell Reports Medicine

Article Title: Dual targeting of lymphocyte homing and retention through α4β7 and αEβ7 inhibition in inflammatory bowel disease

doi: 10.1016/j.xcrm.2021.100381

Figure Lengend Snippet: αEβ7 or E-cadherin blockade inhibits T cell interactions with intestinal epithelium Adoptive transfer of tdTomato + OT-1 cells into WT or E-cadherin-CFP recipients was followed by oral challenge with cholera toxin:OVA the following day. Three days after immunization, recipient mice were treated with isotype or anti-αE or anti-E-cadherin antibodies before analysis. (A) OT-1 cell number in the small intestine lamina propria. Bar graph shows means ± SEM of six to eight mice combined from two independent experiments. (B) Confocal microscopy image of intestinal tissue sections stained with anti-E-cadherin (green) and anti-laminin (blue). White arrows point to tdTomato (red) OT-1 T cells in close proximity to E-cadherin on basolateral epithelium. Scale bar: 100 μm. Data are representative of three independent experiments. (C) Representative movie of tdTomato (red) OT-1 T cells interacting with intestinal epithelium (green) in control or anti-αE-treated mice. The basement membrane is indicated by a dashed white line. Scale bar: 20 μm. (D and E) Maximal track speed (D) and track length (E) were quantified for individual cells. Bar graph shows means ± SEM, and each dot represents an individual cell migration event. Data are representative of five independent experiments. One-way ANOVA with Dunnett’s post-test or unpaired Student‘s t test was used to calculate statistical significance. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

Article Snippet: Anti-αE blocking antibody (clone number M290) was from BioXcell and anti-E-cadherin antibody (clone number ECCD-2) was from Invitrogen.

Techniques: Adoptive Transfer Assay, Confocal Microscopy, Staining, Control, Membrane, Migration

Journal: Immunity

Article Title: Microbiota Sensing by Mincle-Syk Axis in Dendritic Cells Regulates Interleukin-17 and -22 Production and Promotes Intestinal Barrier Integrity

doi: 10.1016/j.immuni.2018.12.020

Figure Lengend Snippet:

Article Snippet: anti-mouse CD103, BUV395, clone M290 , BD Biosciences , Cat#740238; RRID: AB_2739985.

Techniques: Blocking Assay, Negative Control, Recombinant, Staining, Amplification, Enzyme-linked Immunosorbent Assay, PCR Cloning, Software